Myeloid spatial and transcriptional molecular signature of ischemia-reperfusion injury in human liver transplantation.

ABSTRACT:

Background

Ischemia-reperfusion injury (IRI) is a significant clinical concern in liver transplantation, with a key influence on short-term and long-term allograft and patient survival. Myeloid cells trigger and sustain tissue inflammation and damage associated with IRI, but the mechanisms regulating these activities are unknown. To address this, we investigated the molecular characteristics of intragraft myeloid cells present in biopsy-proven IRI- and IRI+ liver transplants.

Methods

RNA-sequencing was performed on 80 pre-reperfusion and post-reperfusion biopsies from 40 human recipients of liver transplantation (23 IRI+, 17 IRI-). We used transcriptional profiling and computational approaches to identify specific gene coexpression network modules correlated with functional

SUBMITTER: Sosa RA 

PROVIDER: S-EPMC10786592 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature