Activation of Ca²⁺ transport in cardiac microsomes enriches functional sets of ER and SR proteins.

ABSTRACT: The importance of sarcoplasmic reticulum (SR) Ca²⁺-handling in heart has led to detailed understanding of Ca²⁺-release and re-uptake protein complexes, while less is known about other endoplasmic reticulum (ER) functions in the heart. To more fully understand cardiac SR and ER functions, we analyzed cardiac microsomes based on their increased density through the actions of the SR Ca²⁺-ATPase (SERCA) and the ryanodine receptor that are highly active in cardiomyocytes. Crude cardiac microsomal vesicles loaded with Ca oxalate produced two higher density subfractions, MedSR and HighSR. Proteins from 20.0 μg of MV, MedSR, and HighSR protein were fractionated using SDS-PAGE, then trypsinized from 20 separate gel pieces, and analyzed by LC-MS/MS to determine prote

SUBMITTER: Cala SE 

PROVIDER: S-EPMC10786961 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature