Characterizing substrate utilization during the fasted state using plasma high-resolution metabolomics.

ABSTRACT:

Objectives

High-resolution metabolomics enables global assessment of metabolites and molecular pathways underlying physiologic processes, including substrate utilization during the fasted state. The clinical index for substrate utilization, respiratory exchange ratio (RER), is measured via indirect calorimetry. The aim of this pilot study was to use metabolomics to identify metabolic pathways and plasma metabolites associated with substrate utilization in healthy, fasted adults.

Methods

This cross-sectional study included 33 adults (mean age 27.7 ± 4.9 y, mean body mass index 24.8 ± 4 kg/m²). Participants underwent indirect calorimetry to determine resting RER after an overnight fast. Untargeted metabolomics was performed on fasted plasma samples using dual-column

SUBMITTER: Taibl KR 

PROVIDER: S-EPMC10787037 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature