Induced expression of the Legionella pneumophila gene encoding a 20-kilodalton protein during intracellular infection.

ABSTRACT: The eukaryotic protein synthesis inhibitor cycloheximid has been used by many investigators to selectively radiolabel intracellular bacteria. Although cycloheximide has no direct effect on bacterial gene expression, there are concerns that long-term inhibition of the host cell protein synthesis may have secondary effects on bacterial gene expression. Therefore, prior to further identification and cloning of the macrophage-induced (MI) genes of Legionella pneumophila, the effects of cycloheximide on L. pneumophila-infected U937 cells were evaluated by transmission electron microscopy. Inhibition of protein synthesis of the host cell for 6 h had no major effect on the ultrastructure of the host cell, on the formation of rough endoplasmic reticulum-surrounded replicative phagosome, or on init

SUBMITTER: Abu Kwaik Y 

PROVIDER: S-EPMC107878 | biostudies-literature | 1998 Jan

REPOSITORIES: biostudies-literature