Expression and characterization of the new antimicrobial peptide AP138L-arg26 anti Staphylococcus aureus.

ABSTRACT: The low activity and yield of antimicrobial peptides (AMPs) are pressing problems. The improvement of activity and yield through modification and heterologous expression, a potential way to solve the problem, is a research hot-pot. In this work, a new plectasin-derived variant L-type AP138 (AP138L-arg26) was constructed for the study of recombination expression and druggablity. As a result, the total protein concentration of AP138L-arg26 was 3.1 mg/mL in Pichia pastoris X-33 supernatant after 5 days of induction expression in a 5-L fermenter. The recombinant peptide AP138L-arg26 has potential antibacterial activity against selected standard and clinical Gram-positive bacteria (G⁺, minimum inhibitory concentration (MIC) 2-16 µg/mL) and high stability under different conditions (t

SUBMITTER: Zhang K 

PROVIDER: S-EPMC10787893 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature