Multiple circulating forms of neprilysin detected with novel epitope-directed monoclonal antibodies.

ABSTRACT: Neprilysin (NEP) is an emerging biomarker for various diseases including heart failure (HF). However, major inter-assay inconsistency in the reported concentrations of circulating NEP and uncertainty with respect to its correlations with type and severity of disease are in part attributed to poorly characterized antibodies supplied in commercial ELISA kits. Validated antibodies with well-defined binding footprints are critical for understanding the biological and clinical context of NEP immunoassay data. To achieve this, we applied in silico epitope prediction and rational peptide selection to generate monoclonal antibodies (mAbs) against spatially distant sites on NEP. One of the selected epitopes contained published N-linked glycosylation sites at N285 and N294. The best antibody pair, m

SUBMITTER: Ling SSM 

PROVIDER: S-EPMC10787894 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature