Need for speed: Human fast-twitch mitochondria favor power over efficiency.

ABSTRACT:

Objective

Human skeletal muscle consists of a mixture of slow- and fast-twitch fibers with distinct capacities for contraction mechanics, fermentation, and oxidative phosphorylation. While the divergence in mitochondrial volume favoring slow-twitch fibers is well established, data on the fiber type-specific intrinsic mitochondrial function and morphology are highly limited with existing data mainly being generated in animal models. This highlights the need for more human data on the topic.

Methods

Here, we utilized THRIFTY, a rapid fiber type identification protocol to detect, sort, and pool fast- and slow-twitch fibers within 6 h of muscle biopsy sampling. Respiration of permeabilized fast- and slow-twitch fiber pools was then analyzed with high-resolution respirometry. Usi

SUBMITTER: Edman S 

PROVIDER: S-EPMC10788296 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature