Distinct lung cell signatures define the temporal evolution of diffuse alveolar damage in fatal COVID-19.

ABSTRACT:

Background

Lung damage in severe COVID-19 is highly heterogeneous however studies with dedicated spatial distinction of discrete temporal phases of diffuse alveolar damage (DAD) and alternate lung injury patterns are lacking. Existing studies have also not accounted for progressive airspace obliteration in cellularity estimates. We used an imaging mass cytometry (IMC) analysis with an airspace correction step to more accurately identify the cellular immune response that underpins the heterogeneity of severe COVID-19 lung disease.

Methods

Lung tissue was obtained at post-mortem from severe COVID-19 deaths. Pathologist-selected regions of interest (ROIs) were chosen by light microscopy representing the patho-evolutionary spectrum of DAD and alternate disease phenotypes were se

SUBMITTER: Milross L 

PROVIDER: S-EPMC10788437 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature