Mucosal recombinant BCG vaccine induces lung-resident memory macrophages and enhances trained immunity via mTORC2/HK1-mediated metabolic rewiring.

ABSTRACT: Bacillus Calmette-Guérin (BCG) vaccination induces a type of immune memory known as "trained immunity", characterized by the immunometabolic and epigenetic changes in innate immune cells. However, the molecular mechanism underlying the strategies for inducing and/or boosting trained immunity in alveolar macrophages remains unknown. Here, we found that mucosal vaccination with the recombinant strain rBCG^PPE27 significantly augmented the trained immune response in mice, facilitating a superior protective response against Mycobacterium tuberculosis and non-related bacterial reinfection in mice when compared to BCG. Mucosal immunization with rBCG^PPE27 enhanced innate cytokine production by alveolar macrophages associated with promoted glycolytic metabolism, typical of tra

SUBMITTER: Peng X 

PROVIDER: S-EPMC10788536 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature