Alterations of Glycan Composition in Aerobic Granular Sludge during the Adaptation to Seawater Conditions.

ABSTRACT: Bacteria can synthesize a diverse array of glycans, being found attached to proteins and lipids or as loosely associated polysaccharides to the cells. The major challenge in glycan analysis in environmental samples lies in developing high-throughput and comprehensive characterization methodologies to elucidate the structure and monitor the change of the glycan profile, especially in protein glycosylation. To this end, in the current research, the dynamic change of the glycan profile of a few extracellular polymeric substance (EPS) samples was investigated by high-throughput lectin microarray and mass spectrometry, as well as sialylation and sulfation analysis. Those EPS were extracted from aerobic granular sludge collected at different stages during its adaptation to the seawater condition

SUBMITTER: Chen LM 

PROVIDER: S-EPMC10788855 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature