Development and clinical application of loop-mediated isothermal amplification combined with lateral flow assay for rapid diagnosis of SARS-CoV-2.

ABSTRACT:

Background

The diagnostic assay leveraging multiple reverse transcription loop-mediated isothermal amplification (RT-LAMP) could meet the requirements for rapid nucleic acid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Methods

The devised assay merged the lateral flow assay with the RT-LAMP technology and designed specific primers for the simultaneous detection of the target and human-derived internal reference genes within a single reaction. An inquiry into the assay's limit of detection (LOD), sensitivity, and specificity was carried out. The effectiveness of this assay was validated using 498 clinical specimens.

Results

This LOD of the assay was determined to be 500 copies/mL, and there was no observed cross-reaction with other respira

SUBMITTER: Tang J 

PROVIDER: S-EPMC10788970 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature