Sequential extraction of RNA, DNA and protein from cultured cells of the same group.

ABSTRACT:

Background

Efficient extraction of nucleic acids and proteins (ENAP) from cells is a prerequisite for precise annotation of gene function, and has become laboratory routine for revealing the mysteries of life. However, cell samples are often from different culture dishes, resulting in inevitable experimental errors and sometimes poor repeatability.

Aim

To explore a method to improve the efficiency of ENAP, minimizing errors in ENAP processes, enhancing the reliability and repeatability of subsequent experimental results.

Methods

A protocol for the sequential isolation of RNA, DNA, and proteins from the same cultured HepG2 cells using RNAzol reagent is presented here. The first step involves culturing HepG2 cells to the exponential phase, followed by the sequential iso

SUBMITTER: Cui YY 

PROVIDER: S-EPMC10789102 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature