Ontology highlight
ABSTRACT:
SUBMITTER: Jiang L
PROVIDER: S-EPMC10789616 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
Jiang Liyun L Yang Jun J He Reqing R Zhu Youlin Y Wang Dong D
STAR protocols 20240105 1
Long non-coding RNAs (lncRNAs) work together with diverse RNA-binding proteins (RBPs) to fulfill key regulations in important cellular functions. Here, we present a protocol to detect lncRNA-RBP interactions in vitro using a tRNA scaffold containing a streptavidin aptamer pull-down assay. We describe steps for preparing both protein and lncRNA transcripts, lncRNA-protein interaction detection with an in vitro binding assay, and western blot analysis. This protocol is applicable to screen for RNA ...[more]