IL-34 attenuates acute T cell-mediated rejection following renal transplantation by upregulating M2 macrophages polarization.

ABSTRACT:

Objective

To investigate the role of Interleukin-34 (IL-34) in acute T cell-mediated rejection (TCMR) following renal transplantation.

Methods

The mice acute TCMR model of renal transplantation was established and identified by hematoxylin and eosin (HE) and immunohistochemistry (IHC) staining. Then, IHC staining of IL-34 was also performed to determine the expression of IL-34 in allografts. Recipients were infected with IL-34 overexpression adeno-associated virus, infection efficiency of which was estimated by enzyme linked immunosorbent assay (ELISA), Western blot, and immunofluorescence. HE and IHC staining were used to estimate the grades of TCMR. Flow cytometry was performed on lymphocytes in spleens of recipients including regulatory T cells (Tregs) and M2 macrophages.

SUBMITTER: Ni B 

PROVIDER: S-EPMC10789621 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature