Structural differences between the closely related RNA helicases, UAP56 and URH49, fashion distinct functional apo-complexes.

ABSTRACT: mRNA export is an essential pathway for the regulation of gene expression. In humans, closely related RNA helicases, UAP56 and URH49, shape selective mRNA export pathways through the formation of distinct complexes, known as apo-TREX and apo-AREX complexes, and their subsequent remodeling into similar ATP-bound complexes. Therefore, defining the unidentified components of the apo-AREX complex and elucidating the molecular mechanisms underlying the formation of distinct apo-complexes is key to understanding their functional divergence. In this study, we identify additional apo-AREX components physically and functionally associated with URH49. Furthermore, by comparing the structures of UAP56 and URH49 and performing an integrated analysis of their chimeric mutants, we exhibit unique structu

SUBMITTER: Fujita KI 

PROVIDER: S-EPMC10789772 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature