Platinum-based drugs induce phenotypic alterations in nucleoli and Cajal bodies in prostate cancer cells.

ABSTRACT:

Purpose

Platinum-based drugs are cytotoxic drugs commonly used in cancer treatment. They cause DNA damage, effects of which on chromatin and cellular responses are relatively well described. Yet, the nuclear stress responses related to RNA processing are incompletely known and may be relevant for the heterogeneity with which cancer cells respond to these drugs. Here, we determine the type and extent of nuclear stress responses of prostate cancer cells to clinically relevant platinum drugs.

Methods

We study nucleolar and Cajal body (CB) responses to cisplatin, carboplatin, and oxaliplatin with immunofluorescence-based methods in prostate cancer cells. We utilize organelle-specific markers NPM, Fibrillarin, Coilin, and SMN1, and study CB-regulatory proteins FUS and TDP-43 usin

SUBMITTER: Batnasan E 

PROVIDER: S-EPMC10790272 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature