Dataset Information
Bile acid fitness determinants of a Bacteroides fragilis isolate from a human pouchitis patient.
Ontology highlight
ABSTRACT: Importance
The Gram-negative bacterium Bacteroides fragilis is a common member of the human gut microbiota that colonizes multiple host niches and can influence human physiology through a variety of mechanisms. Identification of genes that enable B. fragilis to grow across a range of host environments has been impeded in part by the relatively limited genetic tractability of this species. We have developed a high-throughput genetic resource for a B. fragilis strain isolated from a UC pouchitis patient. Bile acids limit microbial growth and are altered in abundance in UC pouches, where B. fragilis often blooms. Using this resource, we uncovered pathways and processes that impact B. fragilis fitness in bile and that may contribute to population expansions during bouts of gut inflammation.
SUBMITTER: Fiebig A
PROVIDER: S-EPMC10790697 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
Publications
Bile acid fitness determinants of a <i>Bacteroides fragilis</i> isolate from a human pouchitis patient.
mBio 20231208 1
<h4>Importance</h4>The Gram-negative bacterium <i>Bacteroides fragilis</i> is a common member of the human gut microbiota that colonizes multiple host niches and can influence human physiology through a variety of mechanisms. Identification of genes that enable <i>B. fragilis</i> to grow across a range of host environments has been impeded in part by the relatively limited genetic tractability of this species. We have developed a high-throughput genetic resource for a <i>B. fragilis</i> strain i ...[more]
