An ALP enzyme-based electrochemical biosensor coated with signal-amplifying BaTiO₃ nanoparticles for the detection of an antiviral drug in human blood serum.

ABSTRACT: Tenofovir (TFV) is an antiviral drug used to treat the co-infections of HIV/HBV viruses. Accurate monitoring of TFV drug levels is essential for evaluating patient adherence, optimizing dosage, and assessing treatment efficacy. Herein, we propose an innovative electrochemical sensing approach by using the alkaline phosphatase (ALP) enzyme with the support of BaTiO₃ nanoparticles. An attractive sensitivity and selectivity of the developed sensor towards TFV detection were achieved. First, the nanoparticles were synthesized by following a single-step sol-gel method and characterized through various analytical techniques, including SEM, EDX, FT-IR, BET, zeta potential, XRD, and UV-vis and Raman spectroscopy. The suggested mechanism demonstrated the formation of a strong bond betwee

SUBMITTER: Draz MU 

PROVIDER: S-EPMC10790964 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature