Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window.

ABSTRACT: 3-Photon microscopy (3PM) excited at the 1700 nm window features a smaller tissue attenuation and hence a larger penetration depth in brain imaging compared with other excitation wavelengths in vivo. While the comparison of the penetration depth quantified by effective attenuation length l_e with other excitation wavelengths have been extensively investigated, comparison within the 1700 nm window has never been demonstrated. This is mainly due to the lack of a proper excitation laser source and characterization of the in vivo emission properties of fluorescent labels within this window. Herein, we demonstrate detailed measurements and comparison of l_e through the 3-photon imaging of the mouse brain in vivo, at different excitation wav

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10790977 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature