A native phosphoglycolate salvage pathway of the synthetic autotrophic yeast Komagataella phaffii.

ABSTRACT: Synthetic autotrophs can serve as chassis strains for bioproduction from CO₂ as a feedstock to take measures against the climate crisis. Integration of the Calvin-Benson-Bassham (CBB) cycle into the methylotrophic yeast Komagataella phaffii (Pichia pastoris) enabled it to use CO₂ as the sole carbon source. The key enzyme in this cycle is ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzing the carboxylation step. However, this enzyme is error prone to perform an oxygenation reaction leading to the production of toxic 2-phosphoglycolate. Native autotrophs have evolved different recycling pathways for 2-phosphoglycolate. However, for synthetic autotrophs, no information is available for the existence of such pathways. Deletion of CYB2

SUBMITTER: Baumschabl M 

PROVIDER: S-EPMC10791038 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature