Exploring metabolic effects of dipeptide feed media on CHO cell cultures by in silico model-guided flux analysis.

ABSTRACT: There is a growing interest in perfusion or continuous processes to achieve higher productivity of biopharmaceuticals in mammalian cell culture, specifically Chinese hamster ovary (CHO) cells, towards advanced biomanufacturing. These intensified bioprocesses highly require concentrated feed media in order to counteract their dilution effects. However, designing such condensed media formulation poses several challenges, particularly regarding the stability and solubility of specific amino acids. To address the difficulty and complexity in relevant media development, the biopharmaceutical industry has recently suggested forming dipeptides by combining one from problematic amino acids with selected pairs to compensate for limitations. In this study, we combined one of the lead amino acids, L-

SUBMITTER: Park SY 

PROVIDER: S-EPMC10791731 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature