Amyloid plaques and normal ageing have differential effects on microglial Ca²⁺ activity in the mouse brain.

ABSTRACT: In microglia, changes in intracellular calcium concentration ([Ca²⁺]_i) may regulate process motility, inflammasome activation, and phagocytosis. However, while neurons and astrocytes exhibit frequent spontaneous Ca²⁺ activity, microglial Ca²⁺ signals are much rarer and poorly understood. Here, we studied [Ca²⁺]_i changes of microglia in acute brain slices using Fluo-4-loaded cells and mice expressing GCaMP5g in microglia. Spontaneous Ca²⁺ transients occurred ~ 5 times more frequently in individual microglial processes than in their somata. We assessed whether microglial Ca²⁺ responses change in Alzheimer's disease (AD) using App^NL-G-F knock-in mice. Proximity to Aβ plaques strongly affected mi

SUBMITTER: Izquierdo P 

PROVIDER: S-EPMC10791787 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature