Two-photon excitation fluorescence in ophthalmology: safety and improved imaging for functional diagnostics.

ABSTRACT: Two-photon excitation fluorescence (TPEF) is emerging as a powerful imaging technique with superior penetration power in scattering media, allowing for functional imaging of biological tissues at a subcellular level. TPEF is commonly used in cancer diagnostics, as it enables the direct observation of metabolism within living cells. The technique is now widely used in various medical fields, including ophthalmology. The eye is a complex and delicate organ with multiple layers of different cell types and tissues. Although this structure is ideal for visual perception, it generates aberrations in TPEF eye imaging. However, adaptive optics can now compensate for these aberrations, allowing for improved imaging of the eyes of animal models for human diseases. The eye is naturally built to filte

SUBMITTER: Kaushik V 

PROVIDER: S-EPMC10791900 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature