Isolation and characterization of novel Bacillus strains with superior probiotic potential: comparative analysis and safety evaluation.

ABSTRACT: Despite the current use of some Bacillus spp. as probiotics, looking for and introducing new efficient and safe potential probiotic strains is one of the most important topics in both microbiology and food industry. This study aimed to isolate, identify, and evaluate the probiotic characteristics and safety of some Bacillus spp. from natural sources. Thirty-six spore-forming, Gram-positive, and catalase-positive Bacillus isolates were identified in 54 samples of soil, feces and dairy products. Bacterial identification was performed using 16S rDNA sequencing. To evaluate the probiotic potential of isolates, the resistance of bacterial cells to simulated gastrointestinal tract (GIT) conditions, the presence of enterotoxin genes, their susceptibility to antibiotics, antimicrobial and hemolyti

SUBMITTER: Golnari M 

PROVIDER: S-EPMC10791968 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature