Frequencies of activated T cell populations increase in breast milk of HCMV-seropositive mothers during local HCMV reactivation.

ABSTRACT:

Background

Human cytomegalovirus (HCMV) can reactivate in the mammary gland during lactation and is shed into breast milk of nearly every HCMV-IgG-seropositive mother of a preterm infant. Dynamics of breast milk leukocytes during lactation, as well as blood leukocytes and the comparison between both in the context of HCMV reactivation is not well understood.

Methods

Here, we present the BlooMil study that aimed at comparing changes of immune cells in blood and breast milk from HCMV-seropositive- vs -seronegative mothers, collected at four time ranges up to two months post-partum. Viral load was monitored by qPCR and nested PCR. Multiparameter flow cytometry was used to identify leukocyte subsets.

Results

CD3⁺ T cell frequencies were found to increase rapidl

SUBMITTER: Lazar K 

PROVIDER: S-EPMC10792025 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature