"Nano COVID-19": Nanopore sequencing of spike gene to identify SARS-CoV-2 variants of concern.

ABSTRACT: Coronavirus disease 2019 (COVID-19) is a worldwide pandemic infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). World Health Organization (WHO) has defined the viral variants of concern (VOC) which cause more severe disease, higher transmissibility, and reduced vaccine efficacy. In this study, the "Nano COVID-19" workflow based on Oxford nanopore sequencing of the full-length spike gene combined with flexible data analysis options was developed to identify SARS-CoV-2 VOCs. The primers were designed to cover the full-length spike gene and can amplify all VOC strains. The results of VOC identification based on phylogenetic analysis of the full-length spike gene were comparable to the whole genome sequencing (WGS). Compared to the standard VOC identifica

SUBMITTER: Nimsamer P 

PROVIDER: S-EPMC10792430 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature