Hydrogel-encapsulation to enhance bacterial diagnosis of colon inflammation.

ABSTRACT: Bacteria can be genetically programmed to sense and report the presence of disease biomarkers in the gastrointestinal (GI) tract. However, diagnostic bacteria are typically delivered via oral administration of liquid cultures, resulting in poor survival and high dispersal in vivo. These limitations confound recovery and analysis of engineered bacteria from GI or stool samples. Here, we demonstrate that encapsulating bacteria inside of alginate core-shell particles enables robust survival, containment, and diagnostic function in vivo. We demonstrate these benefits by encapsulating a strain engineered to report the presence of the biomarker thiosulfate via fluorescent protein expression in order to diagnose dextran sodium sulfate-induced colitis in rats. Hydrogel-encapsulated bacteria engine

SUBMITTER: Aghlara-Fotovat S 

PROVIDER: S-EPMC10792543 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature