Maladaptive lymphangiogenesis is associated with synovial iron accumulation and delayed clearance in factor VIII-deficient mice after induced hemarthrosis.

ABSTRACT:

Background

Mechanisms of iron clearance from hemophilic joints are unknown.

Objectives

To better understand mechanisms of iron clearance following joint bleeding in a mouse model of hemophilia.

Methods

Hemarthrosis was induced by subpatellar puncture in factor VIII (FVIII)-deficient (FVII^-/-) mice, +/- periprocedural recombinant human FVIII, and hypocoagulable (^HypoBALB/c) mice. ^HypoBALB/c mice experienced transient FVIII deficiency (anti-FVIII antibody) at the time of injury combined with warfarin-induced hypocoagulability. Synovial tissue was harvested weekly up to 6 weeks after injury for histological analysis, ferric iron and macrophage accumulation (CD68), blood and lymphatic vessel remodeling (αSMA; LYVE1). Synovial RNA sequencin

SUBMITTER: Cooke EJ 

PROVIDER: S-EPMC10792547 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature