Impact of autophagy inhibition on intervertebral disc cells and extracellular matrix.

ABSTRACT:

Background

Intervertebral disc degeneration (IDD) is a leading contributor to low back pain (LBP). Autophagy, strongly activated by hypoxia and nutrient starvation, is a vital intracellular quality control process that removes damaged proteins and organelles to recycle them for cellular biosynthesis and energy production. While well-established as a major driver of many age-related diseases, autophagy dysregulation or deficiency has yet been confirmed to cause IDD.

Methods

In vitro, rat nucleus pulposus (NP) cells treated with bafilomycin A1 to inhibit autophagy were assessed for glycosaminoglycan (GAG) content, proteoglycan synthesis, and cell viability. In vivo, a transgenic strain (Col2a1-Cre; Atg7 ^fl/fl) mice were successfully generated to

SUBMITTER: Kritschil R 

PROVIDER: S-EPMC10792703 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature