Activation of a non-neuronal cholinergic system in visceral white adipose tissue of obese mice and humans.

ABSTRACT:

Background and objectives

Since white adipose tissue (WAT) lacks parasympathetic cholinergic innervation, the source of the acetylcholine (ACh) acting on white adipocyte cholinergic receptors is unknown. This study was designed to identify ACh-producing cells in mouse and human visceral WAT and to determine whether a non-neuronal cholinergic system becomes activated in obese inflamed WAT.

Methods

Mouse epididymal WAT (eWAT) and human omental fat were studied in normal and obese subjects. The expression of the key molecules involved in cholinergic signaling was evaluated by qRT-PCR and western blotting whereas their tissue distribution and cellular localization were investigated by immunohistochemistry, confocal microscopy and in situ hybridization. ACh levels were measured b

SUBMITTER: Severi I 

PROVIDER: S-EPMC10792749 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature