Epigenetic reshaping through damage: promoting cell fate transition by BrdU and IdU incorporation.

ABSTRACT:

Background

Thymidine analogs have long been recognized for their ability to randomly incorporate into DNA. However, the precise mechanisms through which thymidine analogs facilitate cell fate transition remains unclear.

Results

Here, we discovered a strong correlation between the dosage dependence of thymidine analogs and their ability to overcome reprogramming barrier. The extraembryonic endoderm (XEN) state seems to be a cell's selective response to DNA damage repair (DDR), offering a shortcut to overcome reprogramming barriers. Meanwhile, we found that homologous recombination repair (HRR) pathway causes an overall epigenetic reshaping of cells and enabling them to overcome greater barriers. This response leads to the creation of a hypomethylated environment, which facili

SUBMITTER: Li C 

PROVIDER: S-EPMC10792782 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature