Attenuation of neurovirulence of chikungunya virus by a single amino acid mutation in viral E2 envelope protein.

ABSTRACT:

Background

Chikungunya virus (CHIKV) has reemerged as a major public health concern, causing chikungunya fever with increasing cases and neurological complications.

Methods

In the present study, we investigated a low-passage human isolate of the East/ Central/South African (ECSA) lineage of CHIKV strain LK(EH)CH6708, which exhibited a mix of small and large viral plaques. The small and large plaque variants were isolated and designated as CHIKV-SP and CHIKV-BP, respectively. CHIKV-SP and CHIKV-BP were characterized in vitro and in vivo to compare their virus production and virulence. Additionally, whole viral genome analysis and reverse genetics were employed to identify genomic virulence factors.

Results

CHIKV-SP demonstrated lower virus production in mammalian cells

SUBMITTER: Chen H 

PROVIDER: S-EPMC10792792 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature