Vitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species.

ABSTRACT:

Background

After myocardial infarction, the lost myocardium is replaced by fibrotic tissue, eventually progressively leading to myocardial dysfunction. Direct reprogramming of fibroblasts into cardiomyocytes via the forced overexpression of cardiac transcription factors Gata4, Mef2c, and Tbx5 (GMT) offers a promising strategy for cardiac repair. The limited reprogramming efficiency of this approach, however, remains a significant challenge.

Methods

We screened seven factors capable of improving direct cardiac reprogramming of both mice and human fibroblasts by evaluating small molecules known to be involved in cardiomyocyte differentiation or promoting human-induced pluripotent stem cell reprogramming.

Results

We found that vitamin C (VitC) significantly increased car

SUBMITTER: Fang J 

PROVIDER: S-EPMC10792814 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature