Protein profiling in systemic sclerosis patients with different pulmonary complications using proteomic antibody microarray.

ABSTRACT:

Background

Pulmonary arterial hypertension (PAH) and interstitial lung disease (ILD) are leading causes of systemic sclerosis (SSc)-related death. In this study, we aimed to identify biomarkers for detecting SSc pulmonary complications that are mild and in the early stages to improve the prognosis.

Methods

We screened for serum biomarkers using a proteomic antibody microarray that simultaneously assessed 1000 proteins. Differentially expressed proteins were further verified using ELISA. Finally, we performed a correlation analysis using clinical data.

Results

We identified 125 differentially expressed proteins, of which calcitonin, sclerostin (SOST), CD40, and fibronectin were selected for further verification. Serum calcitonin and SOST levels were significantly eleva

SUBMITTER: Huang J 

PROVIDER: S-EPMC10792928 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature