CRISPRmap: Sequencing-free optical pooled screens mapping multi-omic phenotypes in cells and tissue.

ABSTRACT: Pooled genetic screens are powerful tools to study gene function in a high-throughput manner. Typically, sequencing-based screens require cell lysis, which limits the examination of critical phenotypes such as cell morphology, protein subcellular localization, and cell-cell/tissue interactions. In contrast, emerging optical pooled screening methods enable the investigation of these spatial phenotypes in response to targeted CRISPR perturbations. In this study, we report a multi-omic optical pooled CRISPR screening method, which we have named CRISPRmap. Our method combines a novel in situ CRISPR guide identifying barcode readout approach with concurrent multiplexed immunofluorescence and in situ RNA detection. CRISPRmap barcodes are detected and read out through combinatorial hybridization

SUBMITTER: Gu J 

PROVIDER: S-EPMC10793456 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature