Macrophages undergo functionally significant reprograming of nucleotide metabolism upon classical activation.

ABSTRACT: During an immune response, macrophages systematically rewire their metabolism in specific ways to support their diversve functions. However, current knowledge of macrophage metabolism is largely concentrated on central carbon metabolism. Using multi-omics analysis, we identified nucleotide metabolism as one of the most significantly rewired pathways upon classical activation. Further isotopic tracing studies revealed several major changes underlying the substantial metabolomic alterations: 1) de novo synthesis of both purines and pyrimidines is shut down at several specific steps; 2) nucleotide degradation activity to nitrogenous bases is increased but complete oxidation of bases is reduced, causing a great accumulation of nucleosides and bases; and 3) cells gradually switch to prim

SUBMITTER: John SV 

PROVIDER: S-EPMC10793465 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature