Redox-mediated electrochemiluminescence enhancement for bead-based immunoassay.

ABSTRACT: Electrochemiluminescence (ECL) is a highly sensitive mode of detection utilised in commercialised bead-based immunoassays. Recently, the introduction of a freely diffusing water-soluble Ir(iii) complex was demonstrated to enhance the ECL emission of [Ru(bpy)₃]²⁺ labels anchored to microbeads, but a comprehensive investigation of the proposed 'redox-mediated' mechanism was not carried out. In this work, we select three different water-soluble Ir(iii) complexes by virtue of their photophysical and electrochemical properties in comparison with those of the [Ru(bpy)₃]²⁺ luminophore and the TPrA co-reactant. A systematic investigation of the influence of each Ir(iii) complex on the emission of the Ru(ii) labels on single beads by ECL microscopy reveal

SUBMITTER: Fracassa A 

PROVIDER: S-EPMC10793598 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature