Determining the affinities of high-affinity antibodies using KinExA and surface plasmon resonance.

ABSTRACT: Accurate and efficient affinity measurement techniques are essential for the biophysical characterization of therapeutic monoclonal antibodies, one of the fastest growing drug classes. Surface plasmon resonance (SPR) is widely used for determining antibody affinity, but does not perform well with extremely high affinity (low picomolar to femtomolar range) molecules. In this study, we compare the SPR-based Carterra LSA and the kinetic exclusion assay (KinExA) for measuring the affinities of 48 antibodies generated against the SARS-CoV-2 receptor-binding domain. These data reveal that high-affinity antibodies can be generated straight from selections using high-quality in vitro library platforms with 54% correspondence between affinities measured using LSA and KinExA. Generally, where

SUBMITTER: Erasmus MF 

PROVIDER: S-EPMC10793667 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature