Mt-tRNAs in the polymerase gamma mutant heart.

ABSTRACT:

Introduction

Mice harboring a D257A mutation in the proofreading domain of the mitochondrial DNA polymerase, Polymerase Gamma (POLG), experience severe metabolic dysfunction and display hallmarks of accelerated aging. We previously reported a mitochondrial unfolded protein response (UPT^mt) - like (UPR^mt-like) gene and protein expression pattern in the right ventricular tissue of POLG mutant mice.

Aim

We sought to determine if POLG mutation altered the expression of genes encoded by the mitochondria in a way that might also reduce proteotoxic stress.

Methods and results

The expression of genes encoded by the mitochondrial DNA was interrogated via RNA-seq and northern blot analysis. A striking, location-dependent effect was seen in the expression o

SUBMITTER: Bayazit MB 

PROVIDER: S-EPMC10793997 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature