Analysis of vascular disruption in zebrafish embryos as an endpoint to predict developmental toxicity.

ABSTRACT: Inhibition of angiogenesis is an important mode of action for the teratogenic effect of chemicals and drugs. There is a gap in the availability of simple, experimental screening models for the detection of angiogenesis inhibition. The zebrafish embryo represents an alternative test system which offers the complexity of developmental differentiation of an entire organism while allowing for small-scale and high-throughput screening. Here we present a novel automated imaging-based method to detect the inhibition of angiogenesis in early life stage zebrafish. Video subtraction was used to identify the location and number of functional intersegmental vessels according to the detection of moving blood cells. By exposing embryos to multiple tyrosine kinase inhibitors including SU4312, SU5416, Sor

SUBMITTER: Noth J 

PROVIDER: S-EPMC10794345 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature