Foam fractionation Tags (F-Tags) enabling surfactant-free, activity-preserving recovery of enzymes.

ABSTRACT: Enzymes have become important tools in many industries. However, the full exploitation of their potential is currently limited by a lack of efficient and cost-effective methods for enzyme purification from microbial production. One technology that could solve this problem is foam fractionation. In this study, we show that diverse natural foam-stabilizing proteins fused as F-Tags to β-lactamase, penicillin G acylase, and formate dehydrogenase, respectively, are able to mediate foaming and recovery of the enzymes by foam fractionation. The catalytic activity of all three candidates is largely preserved. Under appropriate fractionation conditions, especially when a wash buffer is used, some F-Tags also allow nearly complete separation of the target enzyme from a contaminating protein. We foun

SUBMITTER: Krause T 

PROVIDER: S-EPMC10794386 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature