A peroxidase-derived ligand that induces Fusarium graminearum Ste2 receptor-dependent chemotropism.

ABSTRACT:

Introduction

The fungal G protein-coupled receptors Ste2 and Ste3 are vital in mediating directional hyphal growth of the agricultural pathogen Fusarium graminearum towards wheat plants. This chemotropism is induced by a catalytic product of peroxidases secreted by the wheat. Currently, the identity of this product, and the substrate it is generated from, are not known.

Methods and results

We provide evidence that a peroxidase substrate is derived from F. graminearum conidia and report a simple method to extract and purify the FgSte2-activating ligand for analyses by mass spectrometry. The mass spectra arising from t he ligand extract are characteristic of a 400 Da carbohydrate moiety. Consistent with this type of molecule, glycosidase treatment of F.

SUBMITTER: Sridhar PS 

PROVIDER: S-EPMC10794396 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature