Integrated analysis of single-cell RNA-seq and chipset data unravels PANoptosis-related genes in sepsis.

ABSTRACT:

Background

The poor prognosis of sepsis warrants the investigation of biomarkers for predicting the outcome. Several studies have indicated that PANoptosis exerts a critical role in tumor initiation and development. Nevertheless, the role of PANoptosis in sepsis has not been fully elucidated.

Methods

We obtained Sepsis samples and scRNA-seq data from the GEO database. PANoptosis-related genes were subjected to consensus clustering and functional enrichment analysis, followed by identification of differentially expressed genes and calculation of the PANoptosis score. A PANoptosis-based prognostic model was developed. In vitro experiments were performed to verify distinct PANoptosis-related genes. An external scRNA-seq dataset was used to verify cellular localization.

SUBMITTER: Dai W 

PROVIDER: S-EPMC10795179 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature