MicroRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development.

ABSTRACT:

Purpose

Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation and may flux into the oviduct. Supplementation of in vitro maturation (IVM) and culture (IVC) medium with extracellular vesicles of FFs supports oocyte maturation and embryonic development. We addressed a hypothesis that miRNA profiles in FFs are crucial background of oocyte maturation and embryonic development.

Methods

FFs were collected from the ovaries of individual cows, and the FFs were classified into Good or Poor FF based on the developmental rate to the blastocyst stage of enclosed oocytes. miRNAs associated with the Good FFs were explored using small RNA sequencing. In addition, FFs were classified using the concentration of Goo

SUBMITTER: Aoki S 

PROVIDER: S-EPMC10795439 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature