Establishment of tumor microenvironment-preserving organoid model from patients with intracranial meningioma.

ABSTRACT:

Background

Although meningioma is the most common primary brain tumor, treatments rely on surgery and radiotherapy, and recurrent meningiomas have no standard therapeutic options due to a lack of clinically relevant research models. Current meningioma cell lines or organoids cannot reflect biological features of patient tumors since they undergo transformation along culture and consist of only tumor cells without microenvironment. We aim to establish patient-derived meningioma organoids (MNOs) preserving diverse cell types representative of the tumor microenvironment.

Methods

The biological features of MNOs were evaluated using WST, LDH, and collagen-based 3D invasion assays. Cellular identities in MNOs were confirmed by immunohistochemistry (IHC). Genetic alteration profile

SUBMITTER: Kim D 

PROVIDER: S-EPMC10795458 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature