Dataset Information

Establishment of tumor microenvironment-preserving organoid model from patients with intracranial meningioma.

ABSTRACT:

Background

Although meningioma is the most common primary brain tumor, treatments rely on surgery and radiotherapy, and recurrent meningiomas have no standard therapeutic options due to a lack of clinically relevant research models. Current meningioma cell lines or organoids cannot reflect biological features of patient tumors since they undergo transformation along culture and consist of only tumor cells without microenvironment. We aim to establish patient-derived meningioma organoids (MNOs) preserving diverse cell types representative of the tumor microenvironment.

Methods

The biological features of MNOs were evaluated using WST, LDH, and collagen-based 3D invasion assays. Cellular identities in MNOs were confirmed by immunohistochemistry (IHC). Genetic alteration profiles of MNOs and their corresponding parental tumors were obtained by whole-exome sequencing.

Results

MNOs were established from four patients with meningioma (two grade 1 and two grade 2) at a 100% succession rate. Exclusion of enzymatic dissociation-reaggregation steps endowed MNOs with original histology and tumor microenvironment. In addition, we used a liquid media culture system instead of embedding samples into Matrigel, resulting in an easy-to-handle, cost-efficient, and time-saving system. MNOs maintained their functionality and morphology after long-term culture (> 9 wk) and repeated cryopreserving-recovery cycles. The similarities between MNOs and their corresponding parental tumors were confirmed by both IHC and whole-exome sequencing. As a representative application, we utilized MNOs in drug screening, and mifepristone, an antagonist of progesterone receptor, showed prominent antitumor efficacy with respect to viability, invasiveness, and protein expression.

Conclusion

Taken together, our MNO model overcame limitations of previous meningioma models and showed superior resemblance to parental tumors. Thus, our model could facilitate translational research identifying and selecting drugs for meningioma in the era of precision medicine.

SUBMITTER: Kim D

PROVIDER: S-EPMC10795458 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Publications

Establishment of tumor microenvironment-preserving organoid model from patients with intracranial meningioma.

Kim Dokyeong D Park Junseong J Park Hyeon-Chun HC Zhang Songzi S Park Minyoung M Park Soon A SA Lee Sug Hyung SH Lee Youn Soo YS Park Jae-Sung JS Jeun Sin-Soo SS Chung Yeun-Jun YJ Ahn Stephen S

Cancer cell international 20240118 1

<h4>Background</h4>Although meningioma is the most common primary brain tumor, treatments rely on surgery and radiotherapy, and recurrent meningiomas have no standard therapeutic options due to a lack of clinically relevant research models. Current meningioma cell lines or organoids cannot reflect biological features of patient tumors since they undergo transformation along culture and consist of only tumor cells without microenvironment. We aim to establish patient-derived meningioma organoids ...[more]

PMID: 38238738

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Dataset Information

Establishment of tumor microenvironment-preserving organoid model from patients with intracranial meningioma.

Background

Methods

Results

Conclusion

Publications

Establishment of tumor microenvironment-preserving organoid model from patients with intracranial meningioma.

Similar Datasets

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