Genome replication in asynchronously growing microbial populations.

ABSTRACT: Biological cells replicate their genomes in a well-planned manner. The DNA replication program of an organism determines the timing at which different genomic regions are replicated, with fundamental consequences for cell homeostasis and genome stability. In a growing cell culture, genomic regions that are replicated early should be more abundant than regions that are replicated late. This abundance pattern can be experimentally measured using deep sequencing. However, a general quantitative theory linking this pattern to the replication program is still lacking. In this paper, we predict the abundance of DNA fragments in asynchronously growing cultures from any given stochastic model of the DNA replication program. As key examples, we present stochastic models of the DNA replication progr

SUBMITTER: Pflug FG 

PROVIDER: S-EPMC10796026 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature