Effect of a sensing charge mutation on the deactivation of KV7.2 channels.

ABSTRACT: Potassium-selective, voltage-gated channels of the KV7 family are critical regulators of electrical excitability in many cell types. Removing the outermost putative sensing charge (R198) of the human KV7.2 shifts its activation voltage dependence toward more negative potentials. This suggests that removing a charge "at the top" of the fourth (S4) segment of the voltage-sensing domain facilitates activation. Here, we hypothesized that restoring that charge would bring back the activation to its normal voltage range. We introduced the mutation R198H in KV7.2 with the idea that titrating the introduced histidine with protons would reinstate the sensing charge. As predicted, the mutant's activation voltage dependence changed as a function of the external pH (pHEXT) while modest changes in the

SUBMITTER: Mehrdel B 

PROVIDER: S-EPMC10796215 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature