Challenges in estimating effective population sizes from metagenome-assembled genomes.

ABSTRACT: Effective population size (N_e) plays a critical role in shaping the relative efficiency between natural selection and genetic drift, thereby serving as a cornerstone for understanding microbial ecological dynamics. Direct N_e estimation relies on neutral genetic diversity within closely related genomes, which is, however, often constrained by the culturing difficulties for the vast majority of prokaryotic lineages. Metagenome-assembled genomes (MAGs) offer a high-throughput alternative for genomic data acquisition, yet their accuracy in N_e estimation has not been fully verified. This study examines the Thermococcus genus, comprising 66 isolated strains and 29 MAGs, to evaluate the reliability of MAGs in N_e estima

SUBMITTER: Wang X 

PROVIDER: S-EPMC10797056 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature