Strengthening the genomic surveillance of Francisella tularensis by using culture-free whole-genome sequencing from biological samples.

ABSTRACT:

Introduction

Francisella tularensis is a highly infectious bacterium that causes the zoonotic disease tularemia. The development of genotyping methods, especially those based on whole-genome sequencing (WGS), has recently increased the knowledge on the epidemiology of this disease. However, due to the difficulties associated with the growth and isolation of this fastidious pathogen in culture, the availability of strains and subsequently WGS data is still limited.

Methods

To surpass these constraints, we aimed to implement a culture-free approach to capture and sequence F. tularensis genomes directly from complex samples. Biological samples obtained from 50 common voles and 13 Iberian hares collected in Spain were confirmed as positive for F. tularensis subs

SUBMITTER: Isidro J 

PROVIDER: S-EPMC10797068 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature