Pain behavior and phenotype in a modified anterior lumbar disc puncture mouse model.

ABSTRACT:

Background

An experimental study was performed to improve the anterior approach model of intervertebral disc degeneration (IVDD).

Objective

The aims of this study were to investigate the anterior approach model of IVDD for the cause of death, phenotypes, and underlying mechanisms of low back pain in mice.

Method

In this study, we conducted an anterior puncture procedure on a cohort of 300 C57BL/6J mice that were 8 weeks old. Our investigation focused on exploring the causes of death in the study population (n = 300) and assessing the time-course changes in various parameters, including radiographical, histological, immunofluorescence, and immunohistochemistry analyses (n = 10). Additionally, we conducted behavioral assessments on a subset of the animals

SUBMITTER: Huang Y 

PROVIDER: S-EPMC10797215 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature